ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
A recombinant E0 gene of bovine viral diarrhea virus protects against challenge with bovine viral diarrhea virus of sika deer
Yugang Gao1, Yajun Wang2, Rui Du1, Quankai Wang1, Pengju Zhang3*, Shuzhi Wang1 and Lianxue Zhang1*
1College of Traditional Material Medicine, Jinlin Agriculture University, ChangChun 130118, P. R. China.
2College of Horticulture, Jinlin Agriculture University, ChangChun130118, P. R. China.
3School of Life Science, Sichuan University, Chengdu 610064, P. R. China.
Accepted 15 January, 2019
Abstract
Eukaryotic expression plasmid (PVAX 1/E0) of E0 gene was constructed from an isolated strain of bovine viral diarrhea virus (BVDV) from sika deer and transfected into the BHK-21 cells to detect E0 gene transcription expression level and protein expression level. It was found that E0 gene could express in BHK-21 cells by RT-PCR and ELESA results. The humoral and cellular immune response levels were detected in rabbits immunized with PAVX1/E0 and inactivated vaccine by ELESA technology, which reached the peak after 42 days immunization and PVAX1/E0 immune rabbits could produce specific higher cells immune responses level in comparison to inactivated vaccine group. This is the first report demonstrating and “immunogenicity” of BVDV E0 gene on “sika deer”, indicating a new vaccine target for BVDV.
Key words: Sika deer, bovine viral diarrhea virus, E0 gene, eukaryotic expression, immune response.