ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 8 (2), pp. 001-007, February, 2019. © International Scholars Journals
Full Length Research Paper
Identification of intense toxoplasmosis in Rural Women in Sudan utilizing distinctive analytic tests
*Mohammed Alek, Deluxe Alkabli and El Mubarak
School of Public Health & Health Informatics, Department of Epidemiology, University of Khartoum, Khartoum, Sudan.
Accepted 20 December, 2018
Abstract
Blood samples from 254 childbearing age women from two villages (EL Nuba and EL Massoudia) were collected after seeking their consent; their written consent form was obtained. Detection of IgM for acute toxoplasmosis was done using two different enzyme-linked immunosorbent assay (ELISA) techniques. Prevalence of IgM was 18.9 and 20.3%; this was obtained by using ELISA IgM in Khartoum for all samples and ELISA IgM in Prague for selected samples, respectively. Diagnostic test was used to confirm acute toxoplasmosis. This consists of detecting IgA and IgE using ELISA technique. The result was the same in both techniques (6.8%). Confirmation tests were used for different purposes. IgG avidity was used to determine the exact time the selected samples had the infection. Six cases had recent infection; five cases had old infection and two cases were in between. Western blot (WB) was used to confirm the antibodies detected by screening and diagnostic tests. Western blot confirmed that 61.5% of samples had antibodies against P30 gene.
Key words: Acute toxoplasmosis, ELISA- IgG avidity, Western blot, rural women, Sudan.