ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-009, March, 2019. © International Scholars Journals
Full Length Research Paper
Biodegradation of swainsonine by five types of plasmid-transformants from genomic library of Arthrobacter sp. HW08
Yanchun Hu1,2, Yan Wang1, Jianna Wang3, Guodong Yang1, Haili Li1, Guoxia Geng1 and Jianhua Wang1
1Department of Internal Medicine, College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China.
2Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Yaan 625014, China.
3Hospital of Northwest A & F University, Yangling 712100, China.
Accepted 11 January, 2019
Abstract
The aim of this study is to analyze the ability of swainsonine (SW) biodegradation by five types of transformants from the genomic library of Arthrobacter sp. HW08, explore preliminarily metabolites and deduce the metabolic pathway. Using 1000 mg·l-1 SW as the sole carbon source, MSM containing five transformants at a proportion for 1:1(V:V) was incubated at 30°C, 180 r·min-1. The degradation rate of SW was 99.78% in 48 h, and SW could be completely degraded in 72 h. Compared with samples containing SW in 0 h, two special spots of metabolites were visualized with iodine vapor by TLC. Two kinds of primary metabolites (stearic acid and palmitinic acid) and two kinds of intermediate metabolites appeared in the process of metabolism of SW by GC and GC-MS whose m/z was 388.2 and 314.3 amu respectively. Ultimately, four kinds of end-products appeared in the process of metabolism of SW. This work demonstrates the preliminary results on metabolites of degrading SW by five transformants from Arthrobacter sp. HW08. This work also provides further information on metabolic pathway of SW from strain HW08.
Key words: Swainsonine, biodegradation, metabolites, transformants, Arthrobacter sp. HW08.