ISSN 2997-1036
International Journal of Hematology | Vol. 10, No. 1, January 2019 | pp. 1–8
DOI: 10.46882/2019/IJH/000109
Original Article
Title: Immunophenotypic profile and clinical stage classification of mature B-cell malignancies utilizing CD200 and CD160 expression variations
Names of Authors: W. A. Adebayo¹, X. Y. Emeka², Z. Z. Salami³
Authors’ Affiliations: ¹Department of Haematology, University of Ibadan, Ibadan, Nigeria; ²Department of Haematology, University of Calabar Teaching Hospital, Calabar, Nigeria; ³Department of Pathology, Lagos University Teaching Hospital, Lagos, Nigeria
Abstract: Multi-color flow cytometry panels play an important role in separating overlapping mature B-cell lymphoproliferative disorders. This prospective study evaluated the diagnostic performance of combining CD200 and CD160 markers to differentiate chronic lymphocytic leukemia from splenic marginal zone lymphoma and hairy cell leukemia in 55 adult patients presenting with persistent absolute lymphocytosis. Lineage markers and monoclonal light chain restriction were established using flow cytometry. Chronic lymphocytic leukemia was confirmed in 38 cases, while 17 were diagnosed with non-CLL mature B-cell variants. Strong, uniform surface co-expression of CD200 and CD160 was detected in 94.7% (36 of 38) of the chronic lymphocytic leukemia cases. In contrast, splenic marginal zone lymphoma cohorts demonstrated a complete absence of CD160 expression alongside dim or negative CD200 profiles (P < 0.001). Hairy cell leukemia variants exhibited bright CD200 paired with negative CD160 parameters. High expression density for both markers correlated with early clinical presentation (Binet Stage A). Incorporating CD200 and CD160 into standard immunophenotyping protocols provides excellent diagnostic specificity, reducing borderline scores and helping classify mature B-cell expansions.
Keywords: Chronic lymphocytic leukemia, CD200, CD160, flow cytometry, lymphoproliferative disorders, immunophenotyping
Manuscript Timeline: Received: October 14, 2018; Revised: November 25, 2018; Accepted: December 10, 2018; Published: January 16, 2019