ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (11), pp. 001-005, November, 2018. © International Scholars Journals
Full Length Research Paper
Prevalence of plasmid-mediated 16S rRNA methylase genes among Proteus mirabilis isolates from a Chinese hospital
Fangyou Yu1, Jingye Pan2, Baixing Ding2, Lehe Yang3, Xueqing Zhang1 and Liangxing Wang3*
1Department of Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou.325000, China.
2Department of Intensive Care Unit, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou.325000, China.
3Department of Respiratory Medicine, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou.325000, China.
Accepted 11 November, 2018
Abstract
16S rRNA methylase-mediated high-level resistance to aminoglycosides has been reported recently in clinical isolates of Gram -negative bacilli from several countries. Five (2.5%, 5/198) of 198 isolates of Proteus mirabilis from a teaching hospital in Wenzhou, China, were positive for 16S rRNA methylase genes (one for armA, four for rmtB) and highly resistant to gentamicin, amikacin and tobramycin (MICs, 256 g/ml). One of five isolates harboring 16S rRNA methyalse genes were extended-spectrum - lactamases (ESBL) producer. The plasmids harboring 16S rRNA methylase genes from four out of five donors were transferred into the recipients, Escherichia coli J53. Among five isolates harboring armA and rmtB, the armA gene and the rmtB genes were located on the plasmids, as determined by Southern hybridization. The present study investigated the prevalence of 16S rRNA methylase genes in clinical isolates of P. mirabilis in China for the first time.
Key words: Proteus mirabilis, 16S rRNA methylase, plasmid.