ISSN 2167-0447
International Journal of Agricultural Sciences Vol. 2 (1), pp. 075-080, January 2012, ISSN: 2167-0447 ©2012 International Scholars Journals
Full Length Research Paper
ELISA-based detection of major potato viruses in tissue culture produced potato germplasm
Muhammad Nasir1*, Syed Sajjid Hussain Zaidi2, Asia Batool3, Mumtaz Hussain1, Babar Iqbal1, Waseem Abbas2 and Muhammad Makky Javed2
1Plant Pathology Research Institute, AARI, Faisalabad, Pakistan.
2Plant Virology Section, Faisalabad, Pakistan.
3Plant Pathology Section, PPRI, Faisalabad, Pakistan.
*Corresponding Author's E-mail: [email protected]
Received December 8, 2011; Accepted January 25, 2012
Abstract
Potato leaves samples of different potato plantlets of three varieties (Cardinal, Desiree, Diamont) produced through tissue culture, were collected from incubation room, glass houses, tunnels (Faisalabad and Murree) and field and tested against major potato viruses through ELISA. 537 out of 6581 samples were infected with different potato viruses. Maximum incidence was of PVX (4.51%) whereas 1.21% of PVY, 0.41% of PLRV, 1.52% of PVS, 0.39% of PVM and incidence of PVA was 0.11% in all tissue cultured potato plantlets. Cardinal variety was infected with PVX (3.21%), PVY (1.27%), PLRV (0.63%), PVS (1.99%); PVM (0.63%) and PVA (0.23%) and Desiree potato variety was infected with PVX (5.90%), PVY (1.52%), PLRV (0.38%), PVS (1.35%); PVM (0.42%) and PVA (0.08%) where as Diamont was infected with PVX (4.30%), PVY (0.80%), PLRV (0.20%), PVS (1.20%) and PVM (0.10%). No virus infection was observed in the samples of all three varieties collected from incubation room. Plantlets collected from glass house, Cardinal variety was infected with PLRV (1.25%), PVS (0.42%) and PVM (0.42%) and plantlets of Desiree infected with PVX (0.75%), PVY (0.75%), PLRV (0.93%), PVS (0.37%) and PVM (0.56%) where as plantlets of Diamont were infected with PVX (1.71%), PVY (0.86%), PLRV (1.14%), PVS (0.57%) and PVM (0.29%). The samples collected from tunnels, Cardinal plantlets were infected with PVX (10.32%), PVY (7.74%), PLRV (2.58%), PVS (12.90%) and Desiree plantlets were infected with PVX (13.92), PVY (4.40) andd PVS (5.86) and plantlets of Diamont were infected with (8.89), PVY (5.93) and PVS (8.89%). The samples collected from Murree tunnels, Cardinal was infected with PVX (2.44%), PLRV (0.28%), PVS (0.70%), PVM (0.42%) and PVA (0.14%) where as samples of Desiree and Diamont were infected with only PVX (4.51%, 3.65% respectively). The samples collected from field, Cardinal variety was infected with PVX (25.00%), PVY (20.00%), PVS (15.00%), PVM (7.50%), PVA (3.75%) and plantlets of Desiree were infected with PVX (44%), PVY (26.67%), PLRV (5.33%), PVS (18.67%), PVM (9.33), PVA (2.67%) and plantlets of Diamont were infected with PVX (34.78%), PVY (8.70%) and PVS (21.74%). These samples were collected from the suspected plantlets and ELISA test was carried out to identify the infecting virus in order to produce healthy potato plantlets for further multiplication of disease free seed potato.
Key words: Potato viruses, symptomatology, ELISA, germplasm, screening.