ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2010
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2010 International Scholars Journals
Full Length Research Paper
Utilization of experimental design and surface response methodology to study the influence of glucose and ammonium sulphate in the chlamydosporulation of Candida albicans FMT123-05
João Vicente Braga de Souza1*, Carolina Talhari2, Dieter Reinel3 and Sinésio Talhari2
1Department of Mycology, Institute of Tropical Medicine of Amazonas, Brazil.
2Department of Dermatology, Institute of Tropical Medicine of Amazonas, Brazil.
3Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
Accepted 07 January, 2010
Abstract
The aim of this work was to evaluate the influence of glucose and ammonium sulphate in the chlamydosporulation of Candida albicans FMT123-05. Different media were produced and inoculated with C. albicans FMT123- 05. The glucose and ammonium sulphate content were defined using an experimental design (22 + star). Glucose content inhibits C. albicans chlamydosporulation two times more than ammonium sulphate. The mathematical model and the surface response demonstrated that the total absence of carbon and nitrogen sources was the best condition for chlamydosporulation of C. albicans 123-05 in the experimental conditions.
Key words: Chlamydosporulation, glucose, ammonium sulphate, experimental design.