ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full length Research Paper
Use of Scopulariopsis acremonium for the production of cellulase and xylanase through submerged fermentation
Loveleen Kaur Sarao*, Maninder Arora and V. K. Sehgal
Department of Processing and Food Engineering, Punjab Agricultural University, Ludhiana 141004, Punjab, India.
Accepted 5 May, 2017
Abstract
Production of cellulase and xylanase by Scopulariopsis acremonium through submerged fermentation was studied using shake flask cultivation media. The effect of process parameters such as effect of temperature, pH and inoculum size was investigated. The enzymes were partially purified using isopropanol and used for enzyme assays. The results indicated that the maximum cellulase and xylanase having an enzyme activity of 694.45 and 931.25 IU, respectively, were produced at 30°C incubation temperature. The pH optimum to achieve these enzyme activities was 5.5 with an inoculum size of 1 x 105 spores ml-1 of tween - 80. Cellulase was deactivated at 80°C while xylanase was deactivated at 70°C.
Key words: Scopulariopsis acremonium, cellulase, xylanase, submerged fermentation.