ISSN 2326-7291
International Journal of Public Health and Epidemiology ISSN 2326-7291 Vol. 14 (2), pp. 001-008, February, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Relationship Between Plasma and Platelet Serotonin Levels in Adolescents with Type 1 Diabetes
Gabriel Manjarrez-Gutiérrez1,2*, Rocío Herrera-Márquez3, Teresa Neri-Gómez1, Jorge Hernández-Rodríguez4
1Laboratory of Molecular Pathology, Cardiology Hospital, National Medical Center (CMN-SXXI), Mexican Institute of
Social Security (IMSS), Mexico City, Mexico
2Medical Research Unit in Neurological Diseases, Specialties Hospital, CMN-SXXI, IMSS, México, DF, México
3Pediatric Hospital, CMN-SXXI, IMSS, Mexico City, Mexico
4Laboratory of Neurontogeny, Department of Physiology, Biophysics and Neurosciences, Center of Research and
Advanced Studies (CINVESTAV) IPN, Mexico City, Mexico.
Accepted 18 September, 2024
Abstract
The aim of this study was to determine, in adolescents with type 1 diabetes, 5-hydroxytryptamine (5-HT, serotonin) levels in plasma and platelets, 5-HT transporter (SERT), platelet 5-HT2A receptor and transglutaminase 2. We performed a cross-sectional studying two groups: 1) adolescents with type 1 diabetes and 2) controls. Three milliliters of blood was obtained from all patients to determine 5-HT levels in plasma and platelets. Furthermore, platelets were obtained to evaluate SERT expression, 5-HT2A receptor and transglutaminase 2 using Western blot. Dot blot was used for 5-HT. Glucose, glycated hemoglobin, albumin, triglycerides, cholesterol, hemoglobin, mean platelet volume and platelet count were also measured. Results were analyzed using Student t and Mann-Whitney U tests; P < 0.05 was accepted as statistically significant. Adolescents with type 1 diabetes had an elevation of plasma glucose and glycated hemoglobin (P < 0.001) as well as an increase the 5-HT levels in plasma and platelets along with a decreased expression of SERT, 5-HT2A and increased transglutaminase 2 in platelets compared to controls (P < 0.05). Increased the 5-HT levels in plasma and platelets, together with the decreased expression of SERT and 5-HT2A receptors and increase in transglutaminase 2 platelets, may have an important role in the prothrombotic state frequently presented by patients with diabetes.
Keywords: Serotonin, type 1 diabetes mellitus, platelets, SERT, 5-HT2A receptor, transglutaminase 2.