ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-011, July, 2018. © International Scholars Journals
Full Length Research Paper
Comparative analysis of antibacterial activities of Xenorhabdus species on related and non-related bacteria in vivo
András Fodor1,2,4*, Andrea M. Fodor2,7, Steven Forst3, Joseph S. Hogan4, Michael G. Klein5, Katalin Lengyel2, Gyula Sáringer1, Erko Stackebrandt6, R. A. J. Taylor5 and Éva Lehoczky1
1Institute of Plant Protection, Georgikon Faculty, University of Pannonia, H-8360 Keszthely, Deák Ferenc utca 17, Hungary.
2Institute of Biology, Departments Genetics and Biochemistry, Faculty of Natural Sciences, Eötvös University, Pázmány Péter sétány 1C, Budapest, Hungary, H-1117; Hungary.
3Department of Biology, Lapham Hall, Milwaukee, WI; University of Wisconsin-Milwaukee, Arizona USA.
4Department of Animal Sciences, The Ohio State University (OSU), OARDC, 1680 Madison Avenue Wooster, Ohio 44691; USA.
5Department of Entomology, OSU, OARDC, 1680 Madison Avenue Wooster, Ohio 44691; USA.
6DSMZ, Deutsche Sammlung von Mikroorganizmen und Zellkulturen GmbH, Braunschweig, Germany.
7MCIC, OSU, OARDC, 1680 Madison Avenue Wooster, Ohio 44691, USA.
Accepted 25 June, 2018
Abstract
Insect-nematode-bacterium mutualistic associations provide attractive systems for discovery of inter kingdom signal compounds and antibiotics. A better understanding of the biological meaning of the inter-specific diversity of compounds with antimicrobial activity of the Steinernema-symbiont Xenorhabdus bacteria may provide options for simultaneous applications in pathogen control. Anti-bacterial activities of representative strains of Xenorhabdus budapestensis, Xenorhabdus szentirmaii, Xenorhabdus innexi, Xenorhabdus ehlersii, Xenorhabdus nematophila, Xenorhabdus bovienii and Xenorhabdus cabanillassii were tested on non-related (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus) bacteria and on each other by previously published bioassays. All active compounds were adsorbed by AmberliteR XAD1180. Chemical and thermal stability of antibacterial factors were determined. Antibiotic factors produced by different Xenorhabdus species against each other differ from those used against other competing bacterial genera. Anti-Xenorhabdus activity of the cell-free medium and sensitivity of the cells of other Xenorhabdus strains negatively correlated in X. innexi and X. bovienii. Some activity remained unchanged during high pressure and 121°C for 10 min. The first comparative analysis of the intraspecific antibacterial activities of Xenorhabdus species demonstrated that some Xenorhabdus species with strong antibacterial activity could be co-cultured and they might be used simultaneously for pathogen control.
Key words: Xenorhabdus, autoclaveable antimicrobials, intra-generic, cross-tolerance.