ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (11), pp. 001-003, November, 2018. © International Scholars Journals
Full Length Research Paper
Nested PCR on semen samples for the detection of Mycobacterium avium subsp paratuberculosis
Ali Sharifzadeh1*, Abbas Doosti2, Mohammad Hashem Fazeli1 and Iman Adavoudi3
1Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
2Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
3Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
Accepted 8 November, 2018
Abstract
Mycobacterium paratuberculosis (MAP) is a gram positive, acid-fast bacterium and cause of Johne’s disease in some animals. The important signs of this disease in bovine are diarrhea, weight loss, bowel inflammation, fever and reduce of milk production. The symptoms of this disease are very similar to Crohn©s disease in humans. The aim of this study was to use nested -PCR as an accurate and fast method to trace MAP in bull semen. Semen samples from 112 bulls were collected and DNA was extracted. Then, nested-PCR was performed by specific primers for IS900 gene of MAP. The PCR products with 230 bp length were estimated as a positive. The frequency of MAP in semen samples were 12.50%. The results were showed nested-PCR is a good procedure with high efficiency for detection of intracellular bacteria such as M. paratuberculosis in bull©s semen samples. Thus, despite this abundance more attention to this disease in bulls to identify MAP quickly is essential.
Key words: MAP, nested-PCR, John’s disease, IS900 gene, semen, bull.