ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN: 2736-1624 Vol. 15 (1), pp. 001-008, January, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Genetic Characterization of Mangosteen (Garcinia mangostana L.) Using SSR Markers
Warid Ali Qosim1*, Sujin Patarapuwadol2 and Kazuo N. Watanabe3*
1) Laboratory of Plant Breeding, Faculty of Agriculture, University of Padjadjaran, Indonesia
2) Center for Agriculture Biotechnology, Kasetsart University, Thailand
3) Gene Research Center, University of Tsukuba, Japan.
Accepted 19 November, 2024
Assessment and utilization of diversity in plant genetic resources is very important to improve of plant species. A mangosteen (Garcinia mangostana L.) seed was formed obligate apomicts process. Genetic diversity of mangosteen was developed by using SSR markers. An inter-simple sequence repeat (ISSR)-suppression - PCR technique established to SSR marker of plant spesies. DNA library construction was used restriction enzyme blunt end Rsa I and adaptor (consist of 48-mer: 5’-GTAATACGACTCACTATAGG GCACGCGTGGTCGACGGCCCGGGCTGGT-3’ and 8-mer with the 3-end capped by an amino residue: 5’-ACCAGCCC-NH2-3). Primer designed by using compound SSR (AC)10; (TC)6(AC)5 or (AC)6(AG)5 and an adaptor primer AP2 and nested PCR using AP1 primers. The PCR product integrated into the plasmid PGEMT Easy Vector System and competence cell Escherichia coli (strain DH5α) and sequence. Eight sequence from sample #G17 with SSR compound (AC)10, (TC)6(AG)5 and (AC)6(AG)5 produced two primer pairs. The primer pairs from sequence positive clone 4 of SSR compound (TC)6(AC)5 were forward primer: 5’-GGCCGTT AAAGTAGCTCAAGAA-3’ and reverse primer:5’-CCGCATAGCATCAGTATCTG TC-3’, while primer pairs from sequence positive clone 10 of SSR compound (AC)6(AG)5 were forward primer: 5’-GTGTTTCCATTTGTTACGCGCT-3’ and reverse primer: 5’TAATGCCGTTGGGCAGTGA-3’.
Keywords: Garcinia mangostana, SSR compound. SSR primer