ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-007, March, 2018. © International Scholars Journals
Full Length Research Paper
Identification and characterization of tenuazonic acid as the causative agent of Alternaria alternata toxicity towards groundnut
P. Suvarnalatha Devi1, M. N. Reddy1, M. Nagalakshmi Devamma2, P. Jayalakshmi1, G. Revathi1 and G. Narasimha3*
1Department. of Applied Microbiology, S.P.Mahila Visvavidyalayam, Tirupati, India.
2Department of Botany, Sri Venkateswara University, Tirupati, India.
3Applied Microbiology Laboratory Department of Virology, Sri Venkateswara University, Tirupati, India.
Accepted 18 January, 2018
Abstract
Alternaria alternata (Fr.) Keissler, the causal agent of Alternariosis of groundnut seeds ( Arachis hypogaea L.) was toxigenic when cultured on various laboratory media. The cell free extract or culture filtrate induced severe chlorosis and necrosis on leaves, inhibition of root and shoot growth of germinating seeds and wilting of seedlings. A phytotoxin was isolated both from cell free extract/culture filtrate and infected tissues, purified and identified as tenuazonic acid, by using thin layer chromatography, ultra violet and infra red spectral analysis. Toxicity was monitored with standard bioassay techniques. The results show patho-physiological significance of tenuazonic acid in disease syndrome.
Key words: Alternaria alternata, groundnut, alternariosis, tenuazonic acid.