ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN: 2167-0404 Vol. 2 (6), pp. 449-456, June, 2013. © International Scholars Journals
Full Length Research Paper
High quality bisulfite sequencing using nanogram amounts of genomic DNA
Jihua Sun1 Honglong Wu1 Guanyu Ji1 Boxin Wu Shujing Yan Wen Gao Rena Lam Wenwei Zhang Xiuqing Zhang*
Beijing Genomics Institute, Shenzhen 518000, China
1These authors contributed equally to this work
*Corresponding author. E-mail: [email protected]
Accepted 5 January, 2013
Abstract
Multiple methods have been developed to decipher the genome-scale DNA methylation (Methylome). Bisulfite sequencing (BS-seq), which combines sodium bisulfite conversion with high throughput sequencing, can measure DNA methylation at single-nucleotide resolutions. However, it normally needs large amounts of genomic DNA (5-10μg) to start with, thus creating an obstacle for it to be widely used. Here we optimized the normal BS-seq method for generating high quality bisulfite sequencing on whole genomes using nanogram DNA (MBS-seq). Systematic comparison the whole genome methylation study based on minute amount of genomic DNA through next generation sequencing technology.
Key words: DNA Methylation, Whole Genome Bisulfite Sequencing, Nanogram Amounts of Genomic DNA.