ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-008, December, 2018. © International Scholars Journals
Full Length Research Paper
Comparative osmotic fragility of three erythrocyte genotypes (HbAA, HbAS and HbSS) of male participants administered with five antimalarial drugs
P. C. Chikezie1*, A. A. Uwakwe2 and C. C. Monago2
1Department of Biochemistry, Imo State University, Owerri, Imo State, Nigeria.
2Department of Biochemistry, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.
Accepted 10 September, 2018
Abstract
IN VIVO study was carried out to ascertain the mean corpuscular fragility (MCF) index and corresponding stability of three erythrocyte genotypes (HbAA, HbAS and HbSS) before (control; t = 0 h) and after (tests; that is, at t = 3, 6 and 18 h) five (5) antimalarial drugs (FansidarTM, HalfanTM, Quinine, CoartemTM, and Chloroquine phosphate) were administered to male participants. Clinically confirmed healthy non-malarious and malarious male participants enrolled for this study. Erythrocytes obtained from these individuals were suspended in two separate sets of Phosphate buffer saline (PBS) solution of decreasing concentrations in the following order: 0.9, 0.7, 0.6, 0.4, 0.3 and 0.2 g / 100 ml. Spectrophotometric method was used to determine the level of erythrocyte osmotic fragility. The mean (±S.D) MCF values of the three genotypes were in the order: HbAAP > 0.05) between the MCF values of HbAA and HbAS erythrocytes. Comparatively, parasitized erythrocytes exhibited significantly (P < 0.05) increased MCF values. The five antimalarial drugs were agents of erythrocyte destabilization in both categories of participants. However, the overall capacities of the drugs to disturb erythrocyte stability diminished as the experimental time progressed.
Key words: Antimalarials, erythrocytes, mean corpuscular fragility, genotypes, osmotic fragility.