ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 14 (5), pp. 001-008, May, 2020. © International Scholars Journals
Full Length Research Paper
High-level acetaldehyde production by an indigenous LACTOBACILLUS strain obtained from traditional dairy products of Iran
Maryam Ghobadi Dana1, Bagher Yakhchali 2*, Ali Hatef Salmanian2 and Ferdous Rastgarjazi Jazii2
1Institute of Standard and Industrial Research of Iran P. O. Box 31585-163, Karaj, Iran.
2National Institute of Genetic Engineering and Biotechnology, Pajhohesh blv. P. O. Box 14965-161, Tehran, Iran.
Accepted 23 March, 2020
Abstract
Isolation and characterization of new LACTOBACILLI strains with high level acetaldehyde production, from traditional fermented milk, in different provinces of Iran, are reported. In this study, a total of 112 local dairy products such as yoghurt and doogh were collected from four provinces in the west of Iran, namely Kordestan, Kermanshah, Ilam and Lorestan. Ninety three isolates with extensive diverse phenotypic and biochemical characteristics were identified. These isolates were screened for productions of acetaldehyde during fermentation of skim milk. 67 isolate were positive for acetaldehyde production. Two strains (KR43 and IL121) with the highest acetaldehyde production were further characterized by amplification and sequencing of 16S rRNA genes and their phylogenetic trees were constructed. The results showed that the two strains were similar to LACTOBACILLUS FERMENTUM. 2-dimensional electrophoresis analysis was used to investigate the proteome of the cytosolic fraction extracted from stationary phase cells of the KR43 strain. Also some spots of KR43 strain were analyzed by MS/MS subunits of acetoin dehydrogenase involved in the production of acetaldehyde from acetoin, which has not been reported in LACTOBACILLI yet. To our knowledge, this is the first report in LACTOBACILLI.
Key words: Acetaldehyde production, acetoin dehydrogenase, Lactobacillus, Iran, proteomics, fermented foods, dairy products.