ISSN 2167-0447
International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 4 (1), pp. 077-082, January, 2014. © International Scholars Journals
Full Length Research Paper
Validation of SSR markers associated with QTLs for pod and kernel traits in groundnut (Arachis hypogaea L.)
B.K. Anitha*, N. Manivannan and C.R. Anandakumar
Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
*Corresponding Author. Email: [email protected]
Accepted 11 October, 2013
Abstract
A set of 64 groundnut genotypes were used to validate reported molecular markers associated with QTLs of pod and kernel traits viz., PM36, PGS19D09 and IPAHM103 for oil content (percent); PM375 for 100-pod weight (g), pod length (mm) and kernel length (mm); PM137, PGS19D09; PM384 for 100-kernel weight (g) and PM45 for days to maturity. Genotype data were generated using 64 genotypes and the seven markers associated with various phenotypic traits. Phenotyping of the studied material was accomplished during post rainy season, 2011-12. Single marker analysis was performed to ascertain the relationship between the marker and traits. The results indicated that markers PM36, IPAHM103 and PGS19D09 for oil content; PM375 for 100-pod weight, pod length and kernel length; PM137 and PM384 for 100-kernel weight explained more than 10 percent of phenotypic variation. In addition, these markers also showed association with other traits. Hence these markers could be considered as a potential tool for marker assisted breeding in groundnut.
Key words: Groundnut, pod and kernel traits, QTLs, single marker analysis, phenotypes.