ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN: 2736-1624 Vol. 15 (2), pp. 001-009, February, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Effects of Cobalt Chloride Stress on Physiological, Biochemical, and Enzymatic Parameters and Protein Profile in Cyamopsis tetragonoloba
*P. Gurusaravanan1, S. Vinoth1, Roy John 1, G. Siva1, N. Boopala Bhagavan2, V. Pandiyarajan3 and N. Jayabalan1
1Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli – 620 024, Tamil Nadu,
India.
2Department of Biotechnology, Sri Kalishwari College, Sivakasi – 626130. Tamil Nadu, India
3Department of Plant Biology and Plant Biotechnology, Ayya Nadar Janaki Ammal College, Sivakasi – 626 124. Tamil
Nadu, India.
Accepted December 14, 2024
We investigated the changes in the morphology, biochemical, antioxidant enzyme activity and protein profiles in Cyamopsis tetragonoliba (L). The crop was treated with different concentrations of CoCl2 (25, 50, 75, 100, 125 µM-1) and the changes in growth, protectively induced oxidative stress in relation to metal stress after 14 days old plant were examined. In this study, the lower concentration of 25 µM cobalt chloride has increased the plant productivity than the other concentrations tested. Chlorophyll and carotenoid pigments decreased significantly in the stressed leaves for their adaptation in stressed condition. The two isozymes CAT 1 and CAT 2 activity were detected from the extract of treated and stress leaves were subjected to polyacrylamide gel electrophoresis. Most prominent peroxidase enzymes were induced by accumulation CoCl2 and possibly resulting in improved effectiveness of the enzyme at high concentration of stressed leaves. The protein profile of cluster bean (Cyamopsis tetragonoliba L.) strongly influenced by severe heavy metal stress (100 µM and 125 µM). It was analyzed through SDS-PAGE and found four new polypeptides, hence it was concluded that more number of new proteins (190 kDa, 54 kDa, 38 kDa and 33 kDa) were synthesized in stress treated plants and it was highly intensive and probably constitutes stress tolerant proteins.
Keywords: Biomass, protein, antioxidants, cluster bean, CoCl2.