ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 5 (4), pp. 409-414, April, 2018. © International Scholars Journals
Full Length Research Paper
A study of local fungal isolate capable of hydrolyzing HEC in the pseudoplastic paint
Amani M. D. El Ahwany1* and Eman A. H. Mohamed2
1Faculty of Science, Botany Department, Alexandria University, Alexandria, Egypt.
2Faculty of Science, Botany Department, Alexandria University (Damanhour Branch), Alexandria, Egypt.
*Corresponding author. E-mail: [email protected].
Accepted 19 July, 2017
Abstract
A pseudoplastic paint manufactured by the Protal Paint Company in Alexandria, Egypt was examined for its quality to resist microbial degradation. The paint was hydrolyzed by extracellular endoglucanase of a local fungal isolate. The main hydrolysed component was the hydroxylethyl cellulose (HEC) thickener. The fungal isolate was identified as Aspergillus niger which showed a DNA monomorphism of 86% to A. niger ATCC 16404 by RAPD. A Plackett–Burman design was performed to determine the factors influencing endoglucanase activity. The analysis revealed the significance of negative effect of glucose concentration on endoglucanase specific activity.
Key words: Hydroxylethyl cellulose, Aspergillus niger, endoglucanase, Plackett-Burman.