ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2003
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2003 International Scholars Journals
Genetic affinities of Fusarim spp. and their correlation with origin and pathogenicity
Mohmed S. Khalil1, Mohmed A. Abdel-Sattar2, Ibrahim N. Aly2, Kamel A. Abd-Elsalam1,3* and Joseph A. Verreet3
1Agricultural Research Center, Plant Pathology Research Institute, Giza, Egypt.
2Suez Canal University, Faculty of Agriculture, Ismailia, Egypt.
3Christian Albrechts Universität zu Kiel, Institut für Phytopathologie, Kiel, Germany.
Accepted 14 April, 2003
Abstract
Random amplified polymorphic DNA (RAPD) analyses was used in combination with pathogenicity assays to study the taxonomic kinships among five Fusarium species. A total of 46 isolates of Fusarium spp. obtained from diseased cotton seedlings showing typical root rot and dampping-off symptoms were characterized. Of 10 primers tested, four primers produced polymorphic amplification patterns with taxon-specific bands, in addition to individual- specific bands. Genetic analysis indicated into 2 main clusters, with the minor cluster included all F. moniliforme and F. solani at the genetic similarity of GS=57.82%. The major cluster consisted of all F. oxysporum, F. avenaceum and F. chlamydosporum clustered at 71% similarity. There was no clear-cut relationship between clustering in the RAPD dendrogram, pathogenicity test and geographic origin of tested isolates. The results suggest that RAPD-PCR is a useful method for analysing genetic variation within and between Fusarium spp.
Key words: DNA-fingerprinting, Fusarium chlamydosporum, genetic homology, RAPD-PCR.