ISSN 2375-1185
African Journal of Agronomy Vol. 1 (3), pp. 046-050, August, 2013. © International Scholars Journals
Full Length Research Paper
Agronomic and molecular evaluation of recombinant inbred lines (RILs) of lentil
C. Bermejo2, V. P. Cravero2, F. S. López Anido1 and E. L. Cointry1*
1Department of Plant Breeding, Rosario National University, (UNR), CC 14, Zavalla S2125ZAA, Argentine.
2CONICET, Zavalla, Argentina.
*Corresponding author. E-mail: [email protected]
Accepted 6 April, 2013
Abstract
Sequence-related amplified polymorphism (SRAP) and morphological markers were studied to compare the efficiency of both marker systems in the evaluation of twenty five lentil (Lens culinaris Medik.) recombinant inbred lines (RILs) and four testers. Data on 13 morphological traits were collected and analyzed. A total of 240 polymorphic SRAP’s bands (76.7%) were scored using four combinations of primers. Cluster analysis and both principal component and principal coordinate analysis were carried out. The entries were grouped in five clusters through procrustes generalized analysis. Relationships among lines revealed by molecular markers were significantly correlated with those based on the agronomic traits (r = 0.75), suggesting that the two systems give similar estimates of genetic relations among the RILs. In future breeding programs parent selections could be based on these traits information in order to broaden the genetic.
Key words: Genetic distance, molecular markers, morphological markers, recombinant inbred lines, SRAP marker.