ISSN 2375-1266
African Journal of Environmental and Waste Management ISSN 2375-1266 Vol. 8 (5), pp. 001-004, May, 2021. © International Scholars Journals
Full Length Research Paper
Potentials of two Trichoderma species as antagonistic agents against Colletotrichum destructivum of cowpea
O. A. Akinbode1* and T. Ikotun2
1Institute of Agricultural Research and Training, I.A.R and T, Ibadan, Nigeria.
2Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria.
Accepted 11 November, 2020
Abstract
Antagonistic potentials of two Trichoderma species that is Trichoderma harzianum and Trichoderma pseudokoningii against the pathogen, Colletotrichum destructivum of cowpea was examined in vitro. Each of the Trichoderma species was paired with the pathogen in 9 cm Petri dishes with Acidified Potato Dextrose Agar (APDA) as the growth medium. Pathogen and antagonists were paired using three methods of pairing. Mycelia radial growth of both the pathogen and the antagonists were taken. The two Trichoderma species significantly inhibited radial growth of the pathogen. Introduction of the antagonists before the pathogen gave the best growth inhibition of C. destructivum. The inhibitory effect of the two Trichoderma species significantly differed from each other at P 0.05. T. pseudokoningii had better inhibition of the mycelia growth of C. destructivum than T. harzianum. T. pseudokoningii and T. harzianum have good antagonistic potentials against C. destructivum.
Key words: Colletotrichum destructivum, Trichoderma pseudokoningii, Trichoderma harzianum, antagonists, inhibition, pathogen.