ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
Parametric optimization of extracellular α-amylase recovery from the fermented bran of Aspergillus awamori MTCC 9997
Kalaiarasi K.1* and Parvatham R.2
1Department of Textiles and Clothing, Avinashilingam University for Women, Coimbatore-43, Tamil Nadu, India.
2Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam University for Women, Coimbatore-43, Tamil Nadu, India.
Accepted 21 January, 2019
Abstract
A newly isolated Aspergillus awamori MTCC 9997 strain from spoiled cooked rice was cultured under solid state using cassava peel powder to produce α-amylase. The effect of extraction medium, solid to solvent ratio, extraction time, temperature and physical state on α-amylase recovery was examined. Among various organic, inorganic solvents and buffer solutions tested, maximum extraction of α-amylase was achieved when phosphate buffer of pH 7.0 was added. A solid to solvent ratio (cassava peel : phosphate buffer) of 1:10 (w/v) resulted in maximum yield of α-amylase. The optimum extraction time was 30 min. At 30°C, α-amylase recovery was found to be the maximum. An appreciable amount of α-amylase was recovered under agitated conditions of the fermented biomass at 150 rpm compared with the quantum recovered under stationary conditions.
Key words: α-Amylase, Aspergillus awamori, solid-state fermentation, extraction.