ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-004, February, 2019. © International Scholars Journals
Full Length Research Paper
In vivo evaluation on Malaysian coastal isolates of Gracilaria changii and Stichopus badionotus through heat-burn methicillin-resistant Staphylococcus aureus (MRSA) infection animal model
Mariana N. S.1,2, Nik K. A. N. I.1,2*, Neela V. K.1, Norfarrah M. A.2 and Zamberi S.1
1Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
2Laboratory of Marine Science and Aquaculture, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Accepted 20 January, 2019
Abstract
Staphylococcus aureus commonly causes bacterial infections such as boils, carbuncles, infections wounds, deep abscesses and bloodstream infections (or bacteraemia). Nowadays, it becomes more difficult to treat such infections due to the appearance of resistant strain known as methicillin-resistant Staphylococcus aureus (MRSA). The objective of this study was to screen on methanolic extracts of seaweed (Gracilaria changii) and local sea cucumber (Stichopus badionotus) for their anti-MRSA activities in vitro and in vivo; by a heat-burn, wound-healing model in rats. Both extracts demonstrated in vitro anti-MRSA activity. The sea cucumber extract demonstrated the same extent of wound healing as did vancomycin at P<0.05. The wound-healing activity of the seaweed extract was superior to that of vancomycin as shown by Tukey’s test (P<0.05). It was concluded that both of these crude extracts have a potential to become an alternative resource for anti-MRSA drugs.
Key words: Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), heat-burn injury, seaweed, sea cucumber, Malaysia.