African Journal of Plant Breeding

ISSN 2375-074X

African Journal of Plant Breeding ISSN 2375-074X Vol. 4 (4), pp. 001-005, November, 2017. © International Scholars Journals

Full Length Research Paper

Resynthesis of Ethiopian mustard (Brassica carinata L.) from related digenomic species: An unexplored possibility

F. A. Sheikh1, S. Najeeb2*, A. G. Rather2 and Shashi Banga3

1,2Shere-e- Kashmir University of Agricultural Sciences and Technology Rice Research and Regional Station Khudwani, Anantnag (J and K)-192102, India.

3Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University Ludhiana -141001, India.

Accepted 22 January, 2017

Abstract

The present study was undertaken to resynthesize Brassica carinata (BBCC, 2n = 34) from its related digenomic species viz. Brassica napus (AACC, 2n=38) and Brassica juncea (AABB, 2n = 36) for generating variability for oil and meal quality. Elite genotypes of B. napus viz. MHO-18-1-184 (“00”) and B. juncea viz. NJHO-3-25(‘0’ C22:1) were involved in interspecific hybridization followed by chromosome doubling, selfing and selection to extract B. carinata type plants without any backcrossing. Morphological and cytological assessment of the hybrids and derived B. carinata was carried out to check the breeding value and genomic stability of derived B. carinata types. The study was successful in developing two (NJ 2 and 3) derived B. carinata type plants, following hybridization between non parental amphiploids viz. B. napus and B. juncea. These derived plants showed zero erucic acid and high oleic acid content (49%), as in the two parental species. The derived B. carinata plants also exhibited sufficient genetic deviation from natural B. carinata and constitute an entirely new source of genetic variability.

Key words: Brassica carinata, interspecific hybridization, fatty acid.