Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-007, February, 2019. © International Scholars Journals

Full Length Research Paper

Biodegradation of 2,2,6,6-Tetramethyl-4-piperidone by three soil bacteria strains and their isolation and identification

Q. F. Li*, L. F. Wang, C. J. Hao, G. M. Zhao and J. H. Wang

College of Veterinary Medicine, Northwest A & F University, Yangling, 712100 Shaanxi, China.

Accepted 12 January, 2019

Abstract

In order to isolate and characterize 2,2,6,6-Tetramethyl-4-piperidone (TMPD)- degrading bacteria and to select the optimal degrading conditions, different bacterial types which can degrade TMPD were isolated from soil samples. Three strains (LF-1, LF-2 and LF-3) showed strong biodegradation ability by UV-visible spectrophotometer analysis. Morphological and biochemical analyses, as well as 16S rRNA gene sequence comparisons of three strains were further carried out. They were identified as Klebsiella oxytoca, Staphylococcus pasteur and Bacillus flexus, respectively. K. oxytoca, S. pasteur and B. flexus had degrading capabilities for TMPD in mineral salt medium of 80.9, 69.8 and 64.9%, respectively, during 24 days of incubation. Maximum degrading rates were obtained by an initial concentration of the TMPD of 200 mg/L, an incubation temperature of 30°C, and constant aeration (180 rev/min). In conclusion, K. oxytoca, S. pasteur and B. flexus show their potentials in TMPD detoxifying and in protecting the TMPD contaminated environment.

Key words: 2,2,6,6-Tetramethyl-4-piperidone (TMPD), biodegradation, Klebsiella oxytoca, Staphylococcus pasteur, Bacillus flexus.