ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 2 (6), pp. 213-216, June, 2015. © International Scholars Journals
Full Length Research Paper
A study of the introduction of β-glucosidase genes into Saccharomyces cerevisiae
S. Parvez*, Z. Mukhtar, F. Rashid, M.I. Rajoka
National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan.
*Corresponding author. E-mail: [email protected].
Accepted 06 February, 2015
Abstract
A β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae. Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicating that the gene is expressed in the bombarded yeast.
Key words: Biolistic transformation, β-glucosidase, Cellulomonas biazotea, Saccharomyces cerevisiae.