ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 8 (11), pp. 001-006, November, 2019. © International Scholars Journals
Full Length Research Paper
The effect of media, oil type and rate on the mycelia wet and dry weights of Lentinus squarrosulus (Mont.) singer and Psathyrella atroumbonata Pegler in submerged liquid culture
Nwanze PI1*, Khan AU2, Ameh JB3 and Umoh VJ4
1Department Of Biological Sciences,College Of Natural And Applied Sciences, Igbinedion University,Okada, P.M.B.0006,Edo State, Nigeria.
2Department Of Biological Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria.
3Department Of Microbiology, Ahmadu Bello University, Zaria. Kaduna State, Nigeria.
4Department Of Microbiology, Ahmadu Bello University, Zaria. Kaduna State, Nigeria.
Accepted 28 July, 2019
Abstract
Lentinus squarrosulus and Psathyrella atroumbonata, two edible indigenous mushroom species, were cultured in four different submerged liquid media supplemented with coconut, cotton, groundnut, butterfat, palm kernel and palm oil at 5 different rates. The differences in the means due to the different media types were highly significant (p<0.01) for the mean mycelia wet and dry weights of both L. squarrosulus and P. atroumbonata. SLCM3, which contained twice the glucose level present in each of the other three liquid culture media, induced the heaviest mean wet and dry mycelia weights of both mushroom species. Oil type had a highly significant (p<0.01) effect on the wet and dry mycelia weights of L. squarrosulus as well as the mycelia wet weight of P. atroumbonata. Butterfat induced the heaviest weights. In addition, oil rate also had a significant effect (p<0.01) on the mean mycelia wet and dry weights of both mushroom species. The oil rates of 0.003, 0.005 and 0.007 ml/ml produced comparable optimum mean mycelia wet and dry weights of the mushrooms.
Key words: Lentinus squarrosulus, Psathyrella atroumbonata, supplemented media, submerged liquid media, oil type and rate.