ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full length Research Paper
Partial characterization of extracellular xylanolytic activity derived from Paenibacillus sp. KIJ1
Inkyung Park2 and Jaiesoon Cho1,2*
1Department of Animal Sciences and Environment, College of Animal Bioscience and Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
2Animal Resources Research Center, College of Animal Bioscience and Technology, Konkuk University, 1 Hwayang- dong, Gwangjin-gu, Seoul 143-701, Korea.
Accepted 28 May, 2016
Abstract
An Antarctic bacterial isolate was found to exhibit the extracellular xylanolytic activity. Based on 16S rRNA gene sequence analysis, the strain was named Paenibacillus sp. KIJ1. The maximum xylanase production was achieved by growing Paenibacillus sp. KIJ1 in media with 0.5% carboxymethylcellulose and 0.5% yeast extract, which were found to be the best sources of carbon and nitrogen, respectively. Optimal enzyme activity occurred at 50°C and pH 6.0. The xylanase was very specific for xylan with little or no activity on other carbohydrates and synthetic aryl-glycosides substrates. Enzyme activity was enhanced by Ca2+, Co2+, Zn2+, Cu2+ and Mn2+ and strongly inhibited by SDS. The KIJ1 xylanase may offer potential for use as a microbial feed additive.
Key words: Paenibacillus sp., antarctic, xylanase, xylan, microbial.