ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (2), pp. 001-010, February, 2018. © International Scholars Journals
Full Length Research Paper
Investigating the effects of various additives on surface activity and emulsification index of biosurfactant resulting from broth media of Bacillus subtilis PTCC 1023
Golamreza Dehghan Noudeh1,3*, Ali Dehghan Noodeh2, Mohammad Hassan Moshafi3, Masoud Ahmadi Afzadi1, Abbas Pardakhti3 and Maryam Salandari3
1Pharmaceutics Research Centre, Kerman University of Medical Sciences, Kerman, Iran.
2Department of Life Sciences, Anglia Ruskin University, East Road, Cambridge, CB1 1PT, UK.
3Department of Pharmaceutics, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Accepted 24 August, 2017
Abstract
Surfactants are amphipathic molecules which reduce surface tension and are widely used in pharmaceutical, cosmetic and food industries. In the present study, the production of biosurfactant by Bacillus subtilis PTCC 1023 was studied. B. subtilis was grown in the nutrient broth medium and biosurfactant production was evaluated by measuring the surface tension and emulsification index (E24) each 24 h. The bacterium’s biosurfactant production was investigated in different status with variable factors such as incubation time, temperature, aeration rate and presence of several additives. Then the best fermentation condition was investigated for maximum biosurfactant production and finally biosurfactant identity was investigated using some chemical and spectroscopy methods. The maximum biosurfactant production by B. subtilis PTCC 1023 was obtained when it was grown in brain-heart broth medium containing FeSO4 (4×10-3 M), MnSO4 (1.3×10-3 M), starch (4%) and castor oil (4%) which incubated in a 300 rpm rotary shaker at 30°C for 24 h. Lipopepetide natures in this biosurfactant was confirmed by biochemical and spectroscopic methods.
Key words: Bacillus subtilis, biosurfactant, emulsification index, surface tension.