ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN: 2736-1624 Vol. 15 (2), pp. 001-008, February, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full length Research Paper
Thermo-alkali Stable Catalase from Bacillus sp.: Purification and Biochemical Characterization
Asmaa Ali Hussein
Department of Biotechnology, College of Science, University of Al-Nahrain, Baghdad, Iraq. E-mail: [email protected]
Accepted December 14, 2024
Thermo-alkali stable catalases are promising enzymes in biotechnological applications as H2O2-detoxifying systems. Catalase has been purified and characterized in this study from BACILLUS sp. The purification was performed with a three step procedure consisting of ammonium sulfate precipitation, ion exchange chromatography on DEAE- cellulose and gel filtration chromatography on Sephacryl-S-300 and finally achieved a 16.6-fold-purifying over the crude extract with 50% overall yield. The purified catalase evidenced an estimated molecular weight of 65 kD. The enzyme also exhibited a broad optimal pH (6.0-10.0), and remained stable (100%) in alkaline pH (8.0-10.0),also it was most active at 70°C and stable within a range of 50-80°C. 2- mercaptoethanol, sodium azide, and potassium cyanide are known protein inhibitors, inhibited catalase activity by 70, 50 and 35% respectively.
Keywords: Purification, characterization, thermo-alkali stable, catalase, bacillus sp.