ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
In vitro antimicrobial studies of Nodulisporium specie: An endophytic fungus
S. Rehman1*, Tariq Mir2, A. Kour1, P. H. Qazi1, P. Sultan1 and A. S. Shawl1
1Indian Institute of Integrative Medicine (CSIR), Sanatnagar, Srinagar, India.
2Maternity and Child Hospital, Dammam, India
Accepted 5 April, 2011
Abstract
Nodulisporium sp. an endophytic fungus identified by 28s ribosomal gene sequencing isolated from a medicinal plant, Nothapodytes foetida was studied for its in vitro antimicrobial activity. Dual culture studies were carried out for antifungal activity where maximum antagonistic activity was against Alternaria alternata and Colletotrichum gleosporoides. For antibacterial studies, Gram positive and Gram negative human pathogens strains were used. The minimum inhibitory concentration (MIC) of ethyl acetate and methanol fractions of Nodulipsporium showed appreciable growth inhibition mainly active against disease causing Gram positive bacteria.
Key words: Nothapodytes foetida, endophytes, antagonism, antimicrobial, Nodulisporium, sp., pathogen, bacteria, minimum inhibitory concentration.