ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN 7295-2849 Vol. 8 (9), pp. 001-005, September, 2018. © International Scholars Journals
Full Length Research Paper
Bamboo species relations revealed by random amplified polymorphism chloroplast DNA
H. Y. Zhang1, Y. M. Yang2 and X. Z. Liu1*
1Key Laboratory of Biodiversity Conservation in Southwest China, State Forest Administration, Southwest Forestry University, White Dragon Temple, Kunming, Yunnan Province-650224, Peoples Republic of China.
2Bamboo and Rattan Research Institute, Southwest Forestry College, White Dragon Temple, Kunming, Yunnan Province-650224, Peoples Republic of China.
Accepted 24 April, 2018
Abstract
The polymorphism, similarities and relationships among bamboo species were assessed with random amplified polymorphism chloroplast DNA (RAPD) analysis. The results showed distinct chloroplast DNA differences, present among the species. 175 bands were detected of which 94 were polymorphic (53.1%).3 to 15 bands generated by a single primer of variable lengths were detected, with an average of 7.2 polymorphic bands. The genetic similarity coefficients ranged from 0.543 to 0.954, and most of them were <0.75. Meantime, species relationships were estimated through Unweighted Pair Group Method with Arithmetic Mean (UPGMA) analysis based on RAPD data. It is a useful method for analysis of the relationship in bamboo species.
Key words: Bamboo species, chloroplast DNA, relationships, random amplified polymorphism chloroplast DNA (RAPD), unweighted pair group method with arithmetic mean (UPGMA).