ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (6), pp. 001-005, June, 2018. © International Scholars Journals
Full Length Research Paper
Antimicrobial susceptibility pattern of shiga toxin-producing Escherichia coli strains
Everlon Cid Rigobelo1*, Renato Pariz Maluta2 and Fernando Antonio de Ávila3
1Campus Experimental de Dracena- UNESP, Brazil.
2Departamento de Patologia Veterinaria, Faculdade de Ciencias Agrarias e Veterinarias de Jaboticabal, Brazil.
3Faculdade de Cencias Agrarias e Veterinarias de Jaboticabal. Programa de Pos-Graduacao em Microbiologia Agropecuaria, Brazil.
Accepted 23 April, 2018
Abstract
Shiga toxin -producing Escherichia coli (STEC) is recognized wordwide as human pathogen. During the processing of carcasses, fecal contamination or transfer of bacteria from the animals’ hide to carcasses can promote transmission of pathogenic E. coli to food supplies. A survey was performed to determine the sensibility profile to several antimicrobial drugs of STEC in carcasses obtained from an abattoir in Brazil between March 2008 and August at 2009. A total of 120 STEC were isolated. All isolates were confirmed as being E. coli by their biochemical analysis and submitted to Polymerase Chain Reaction (PCR) for detection of stx, eae and ehly genes. The most frequent resistance was seen against cephalothin (84.0%), streptomycin (45.0%), nalidixic acid (42.0%) and tetracycline (20.0%). Multidrug resistance (MDR) to three or more antimicrobial agents was observed in 46 (38.3%) E. coli isolates. The presence of STEC and MDR strains among the isolates in the beef carcasses emphasizes the importance of proper handling to prevent carcass contamination.
Key words: Escherichia coli, multi-drug resistance, shiga toxin-producing E. coli.