ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Detection of adhesin genes and slime production among Staphylococci in orthopaedic surgical wounds
Nizami Duran1*, Yunus Dogramaci2, Burcin Ozer1, Cemil Demir1 and Aydiner Kalaci2
1Department of Microbiology and Clinical Microbiology, Medical Faculty, Mustafa Kemal University, Hatay, Turkey.
2Department of Orthopaedics and Traumatology, Medical Faculty, Mustafa Kemal University, Hatay, Turkey.
Accepted 12 March, 2016
Abstract
This study was aimed at investigating: (i) three adhesin genes (clf A, fnb A and cna) in Staphylococus aureus strains, (ii) the presence of slime (ica A and ica D genes) in both Staphylococus epidermidis and S. aureus strains isolated from surgical wounds. The slime and adhesin genes were detected by multiplex PCR. The ica A/ica D positivity rates were determined as 66.2% (104/157) in a total of 157 staphylococcal strains. While the occurance rate of slime genes was 69.6% (48/69) among the S. epidermidis, this ratio was 63.6% (56/88) among the S.aureus isolates. No statistically significant difference was found between S. epidermidis and S. aureus isolates in terms of the presence of slime genes (p > 0.05). Among the 88 S. aureus strains, almost all of the strains were positive for fnb A gene (97.7%). The cna and clf A positivity rates were detected in 69 (78.4%) and 45 (51.1%) isolates, respectively. The ica A and ica D genes responsible for slime production have been found to have high prevalence. Also, the frequency of adhesin genes was determined at a high rate in S. aureus strains isolated from surgical wounds. Molecular identification of virulent staphylococcal strains may help in management in clinical decision making.
Key words: Adhesins, slime, Staphylococus aureus, Staphylococus epidermidis, wound infection.