ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Antimicrobial resistant pattern of Escherichia coli from human clinical samples in Osogbo, south western Nigeria
Olowe O. A1, Okanlawon B. M2, Olowe R. A3 and Olayemi A. B4
1Department of Medical Microbiology and Parasitology, P.M.B.4400, Ladoke Akintola University of Technology, College
of Health Sciences, Osogbo, Osun State, Nigeria.
2Department of Biomedical Sciences, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology,
College of Health Sciences, Osogbo, Osun State, Nigeria.
3Department of Biology, Federal University of Technology, Akure Ondo State, Nigeria.
4Department of Microbiology, Faculty of Sciences, University of Ilorin, Kwara State, Nigeria.
Accepted 17 January, 2012
Abstract
We screened 211 clinical samples of which total of 135 Escherichia coli isolates from different human clinical specimens comprising urine, stool, wound swabs, high vaginal swabs, ear swabs and blood obtained from patients at Ladoke Akintola University Teaching Hospital, Osogbo, Osun State, Nigeria. The isolated E. coli were screened for their antibiograms and plasmid profiles. Seven antimicrobial drugs were used during the study. The prevalence of strains resistance to antimicrobials were; Tetracycline (91.6%), Ampicillin (86.7%), Sulphnamide (77.8%) and Gentamicin and Nalidixic acid which were (39.3%) and (4.1%) respectively. A total of seven antibiotic resistance profiles were obtained with over 64% of the isolates showing multi-drug resistance. Plasmids of three size ranges were detected in all of the isolates. Isolates with high multi-drug resistance profiles were found to possess multiple plasmids with large sizes in the range < 6 – 25 kb. Very large resistance levels > 85% were detected against Tetracycline, Sulphnamide, and Cotrimoxazole while Nalidixic acid showed least resistance of 4.1% among the isolates. Majority of the isolates were positive for betalactamase production when subjected to starch paper method.
Key word: Escherichia coli, betalactamse, antibacteria resistance.