Antibacterial activity and mechanism of recombinant human - defensin 5 against clinical antibiotic-resistant strains

Advanced Journal of Microbiology Research Vol. 2016

Available online at http://internationalscholarsjournals.org/journal/ajmr

Full Length Research Paper

Antibacterial activity and mechanism of recombinant human-defensin 5 against clinical antibiotic-resistant strains

A. P. Wang, Y. P. Su, S. Wang, M. Q. Shen, F. Chen, M. Chen, X. Z. Ran, T. M. Chen and J. P. Wang*

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury of PLA, Third Military Medical University, Chongqing, 400038, People’s Republic of China.

Accepted 1 March, 2016

Abstract

The increasing clinical bacterial strains resistant to conventional antibiotics have being a great challenge to the public’s health. As a novel kind of antimicrobial agent, defensins are undoubtedly worthy of exploitation for the treatment of antibiotic-resistant bacteria. To evaluate the antibacterial potency of recombinant mature human -defensin 5 (rmHD₅) against clinical pathogenic strains, we examined its antibacterial kinetics and bactericidal efficacy on forty-nine bacterial strains (belonging to eleven species) with different antibiotic-resistant phenotypes, isolated from digestive and urogenital tracts of the inpatient. Meanwhile, the action mechanism of rmHD₅ was analyzed by transmission electron microscopy observation and membrane permeability detection. The peptide of rmHD₅ was found to possess high potency against all the tested isolates at concentrations of 6 - 12 g/ml for gram-negative (G^-) bacteria and 28 - 32 g/ml for gram-positive (G⁺) bacteria. G^- bacteria were more susceptible to the peptide than G⁺ bacteria. Abnormal morphological changes and increased permeabilization of the cytomembrane were observed in both G ^- bacteria and G⁺ bacteria treated with rmHD₅. The antibacterial activity of rmHD₅ may be tightly associated with the biomembrane permeabilization. Recombinant mHD₅ is a promising candidate to be developed into therapeutic agents for bacterial infections

Key words: Antibacterial activity, mechanism, antibiotics resistant strain, human alpha defensin 5.

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Y. P. Su on Google Scholar Y. P. Su on Pubmed T. M. Chen and J. P. Wang* on Google Scholar T. M. Chen and J. P. Wang* on Pubmed F. Chen on Google Scholar F. Chen on Pubmed S. Wang on Google Scholar S. Wang on Pubmed M. Chen on Google Scholar M. Chen on Pubmed X. Z. Ran on Google Scholar X. Z. Ran on Pubmed A. P. Wang on Google Scholar A. P. Wang on Pubmed M. Q. Shen on Google Scholar M. Q. Shen on Pubmed