ISSN 2167-0404
International Journal of Medicine and Medical Sciences ISSN 2167-0404 Vol. 5 (2), pp. 188-201, March, 2015. © International Scholars Journals
Full Length Research Paper
Biochemical and hematological indices of normal human subjects, pre-treatment, and combined therapy (semi-vegan diet and metformin)-administered diabetic patients of Imo State University Teaching Hospital Orlu, Nigeria
*Obimba, Kelechukwu Clarence., 1Alisa, Christopher O, 2Ozougwu, Jevas Chibuike, 3Nwakamma, Gerald and 4Nwakwudo, Eucharia N.
*,4Department of Biochemistry, School of Science, Federal University of Technology Owerri, Nigeria.
1Department of Chemistry, School of Science, Federal University of Technology Owerri, Nigeria.
2Physiology and Biomedical Research Unit, Department of Biological Sciences, College of Basic and Applied Sciences, Rhema University Aba, Abia State, Nigeria.
3Department of Optometry, School of Health Technology, Federal University of Technology Owerri. Imo State. Nigeria.
E-mail: [email protected]
Accepted 10 March, 2015
Abstract
The aim of the study is to investigate the efficacy of the use of biochemical, and hematological indices as diagnostic parameters of type 2 diabetes mellitus disease, useful for monitoring recovery from the disease. The experimental units were twenty (n=20) pre-treatment diabetic patients (pre-treat D patients); the same twenty pre-treat D patients administered with metformin (glucophage: dose ≈1250 mg/day) and balanced semi-vegan diet (met-AD patients) for a six-week treatment period; and twenty normal human subjects (Norm-H subjects). The mean values (± standard error) of fasting blood sugar (FBS), serum glycated albumin (GA), fasting serum insulin (I), serum aspartate aminotransferase (AST), serum cholesterol (C), serum triglyceride (TG), glycated hemoglobin (HbAc1), serum Ca2+ were significantly higher (p<0.05), (p<0.01, for Ca2+), in the pre-treat D patients compared with the met-AD patients (73.98±1.15mg/dl , 15.8±0.5%, 8.2 ±0.9 mU/L, 9.9 ±1.0 U/l, 172.5 ± 11 mg/dl, 80.1±1.5 mg/dl, 5.25±0.8%, and 2.29±0.10 mmol/l, respectively) and Norm-H subjects. The mean values of Mg2+ and Na+ were significantly lower (p<0.01) in the pre-treat D patients compared with the met-AD patients (0.99±0.05 mmol/l and 142.5±0.2 mmol/l), respectively, and Norm-H subjects. Multiple regression studies revealed that FBS regressed significantly (p<0.05) with C, TG, GA, and HbAc1 of met-AD patients. The correlation statistical analysis between C and TG of pre-treatment D patients was significant (p<0.05), r = 0.985. Analyzed indices are effective and sufficient for the diagnosis, and post-treatment monitoring of recovery from type 2 diabetes mellitus. Prognosis studies show that a combined semi-vegan dietary and drug (metformin/glucophage) therapy is very efficient and useful in the management and treatment of type 2 diabetes mellitus.
Key words: Serum, glycated, treatment, prognosis, rehabilitation.