ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (3), pp. 001-009, March, 2018. © International Scholars Journals
Full Length Research Paper
Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran
Kumarss Amini1, Taghi Zahraei Salehi1*, Gholamreza Nikbakht2, Reza Ranjbar3, Javid Amini4 and Shahrnaz Banou Ashrafganjooei5
1Department of Microbiology, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and Research Branch, Tehran, Iran.
2Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
3Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
4Department of Microbiology, Islamic Azad University of Kerman, Iran.
5Department of Microbiology, Kerman University of Medical Science, Kerman, Iran.
Accepted 23 January, 2018
Abstract
It is important to study the genotypic diversity of Salmonella plasmid genes which are responsible for its virulence. In the present study multiplex polymerase chain reaction (multiplex PCR) assay was carried out for detection of Salmonella enteritidis and presence of invA and spv genes. In the first stage of the study, 1001 poultry samples were collected from a slaughterhouse in Kerman province (southern Iran). Biochemical and serological tests were then performed for identification of Salmonella serovars and 6.79% (68/1001) were positive for Salmonella. Multiplex PCR with three set primers was then applied to confirm serovar enteritidis 51.4% (35/68). Simple-PCR was then applied to detect spvA (Salmonella plasmid virulence), and spvB genes. Finally, multiplex PCR assay was carried out to simultaneously detect and identify invA and spvC genes. The presence of spvA, spvB and spvC in S. enteritidis was 88.6% for each gene. In the second stage of the study, thirty-three bovine (n = 13) and human (n = 20) S. enteritidis strains were isolated from the culture collection in the Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Iran. The analyses of the samples revealed that spvA, spvB, and spvC genes were present in 90% of S. enteritidis from human sources as compared to 100% in bovine sources. The study represents the first report in Iran about the genotypic diversity of spvA, spvB and spvC genes of S. enteritidis.
Key words: Salmonella enteritidis, multiplex PCR, virulence genes.