ISSN 2756-3685
International Journal of Enology and Viticulture Vol. 5 (6), pp. 001-005, June, 2018. © International Scholars Journals
Full Length Research Paper
Antibacterial action of an aqueous grape seed polyphenolic extract
Veronica Sanda Chedea1,5*, Cornelia Braicu2, Flore Chirilǎ3, Ciprian Ober4 and Carmen Socaciu1
1Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania.
2Cancer Institute “I. Chiricuta” Cluj-Napoca, 34-36 Republicii Str., Cluj-Napoca, Romania.
3Depatment of Microbiology-Immunology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania.
4Department of Anesthesiology and Surgical Propedeutics, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania.
5Laboratory of Animal Biology, National Research Development Institute for Animal Biology and Nutrition (IBNA), Calea Bucuresti 1, 077015 Balotesti, Romania.
Accepted 25 March, 2018
Abstract
The potential of a polyphenolic grape seed extract for use as a natural antibacterial agent was evaluated. Pure catechin (CS) and a previously LC-MS characterized grape seed phenolic extract (PE) were evaluated as antibacterial agents against ESCHERICHIA COLI and BREVIBACTERIUM LINENS on solid and in liquid culture media. After 48 h incubation on solid medium, PE had a bactericidal effect on the gram positive B. LINENS and a reduction of the microbial growth for E. COLI. The antibacterial agents tested were effective against E. COLI for 13 h, after 7 h incubation, but ineffective against B.LINENS in liquid medium. CS and PE both had an antibacterial effect depending on incubation time.
Key words: Catechin, polyphenolic extract, antibacterial activity, Brevibacterium linens, Escherichia coli B41.