ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-003, December, 2018. © International Scholars Journals
Short Communication
Differentiation of modern and ancestral Mycobacterium tuberculosis in Northwest region of Iran by screening for the presence of TbD1
Mohammad Asgharzadeh1*, Hossein Samadi Kafil2, Kazem Najati3 and Khalil Ansarin1
1Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2Department of Medical Bacteriology, School of Medicine, Tarbiat Modares University, Tehran, Iran.
3Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Accepted 25 August, 2018
Abstract
Mycobacterium tuberculosis is responsible for considerable human morbidity and mortality worldwide. Based on the presence or absence of M. tuberculosis specific deletion (TbD1), M. tuberculosis isolates are divided into ancestral and modern strains. The aim of this study was to differentiate ancestral and modern M. tuberculosis in northwest of Iran. 165 M. tuberculosis strains were isolated from West and East Azarbaijan provinces of Iran. Ancient and modern M. tuberculosis isolates were distinguished by primer specific polymerase chain reaction (PCR). This study showed that 49 (29.7%) of the isolates were modern M. tuberculosis and 116 (70.3%) were ancestral M. tuberculosis. The prevalence of modern M. tuberculosis in West Azarbaijan was relatively higher than that of East Azarbaijan. Considering the increasing rate of modern M. tuberculosis in the studied region and in world, which has resulted in multi-drug resistance and low preventive effect of bacillus of calmette and guerin (BCG) vaccine, the fast diagnosis, prevention, treatment and more controlling programs of the infection, is important in this region.
Key words: TbD1, modern tuberculosis, ancestral tuberculosis, PCR.