ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Effect of 50 Hz electromagnetic fields on acid phosphatase activity
K. S. Prashanth1*, T. R. S. Chouhan2 and Snehalatha Nadiger3
1Research Scholar, Dr M.G.R University, Chennai Tamil Nadu, India.
2Ethica Matrix CRO Pvt Ltd, Hyderabad, India.
3Department of Biotechnology, New Horizon College of Engineering, Outer Ring Road, Panathur post Bangalore-560087, Karnataka(s) India.
Accepted 29 January, 2017
Abstract
The effect of extremely low frequency (ELF) electromagnetic field (EMF) (50 Hz 0.5 mT) on the activity of acid phosphatase (EC 3.1.3.2) was studied. In addition the factors affecting the enzyme activity such as the temperature, pH and substrate concentration were also investigated. The results show that ELF EMF have significant influence on enzyme activity. Upon EMF exposure Km increased from 0014 ± 0.005 to 0.040 ± 0.008 mM whereas Vmax increased from 0.991 ± 0.254 to 1.638 ± 0.345 µmol/min. Further studies can probably help in finding suitable applications for ELF based modulation of enzyme activity.
Key words: ELF EMF, acid phosphatase, enzyme activity.