ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-003, March, 2019. © International Scholars Journals
Short Communication
Preparation of rabbit anti-Ganoderma sinensis immunomodulatory protein polyclonal antibody
Qizhang Li1,2, Shengbo Zheng1, Xuefei Wang1, Tingwen Bao3 and Xuanwei Zhou1*
1Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China.
2Shanghai Grain Science Research Institute, Shanghai 200136, People’s Republic of China.
3School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China.
Accepted 09 January, 2019
Abstract
Ganoderma sinensis immunomodulatory protein (FIP-gsi) was a new protein in fungal immunomodulatory protein (FIP) family. Based on the recombinant FIP-gsi expressed in Escherichia coli, the New Zealand white rabbits were immunized with the purity protein to prepare anti-FIP-gsi polyclonal antibody. The efficacy of polyclonal antibody was detected by ELISA and Western blot. The results showed that the anti-FIP-gsi polyclonal antibody with high efficient value and specificity has been successfully preparation, and its efficient value was 1:625,000 detected by indirect ELISA, and a special band had been observed by Western blot method. This study established a method to identify FIP-gsi by immunoblotting, and will lay a foundation for further exploring the immunologic function of FIP-gsi.
Key words: Ganoderma sinensis fugal immunomodulatory proteins (FIP-gsi), polyclonal antibody, ELISA, Western blot.