ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Comparison of extract bio-activities of in-situ and in vitro grown selected bryophyte species
Aneta Sabovljevi 1, Marina Sokovi 2, Jasmina Glamo lija3, Ana iri 2, Milorad Vuji i 1, Boris Pejin3 and Marko Sabovljevi 1*
1Faculty of Biology, Institute of Botany and Botanical Garden, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia.
2Institute for Biological Research “S. Stankovic”, Bul. Despota Stefana 142, 11000 Belgrade, Serbia.
3Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
Accepted 26 February, 2015
Abstract
The antimicrobial activity of DMSO extracts of three bryophyte species, two mosses and a liverwort (Atrichum undulatum (Hedw.) P. Beauv., Marchantia polymorpha L. ssp. ruderalis Bischl. and Boisselier, Physcomitrella patens (Hedw.) Bruch and Schimp.) grown in nature and in axenic culture was evaluated by microdilution method against eight bacterial species ( Escherichia coli ATCC 35210, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311, Enterobacter cloacae (human isolate), Listeria monocytogenes NCTC 7973, Bacillus cereus (human isolate), Micrococcus flavus ATCC 10240 and Staphilococcus aureus ATCC 6538). All investigated bryophyte extracts are proved to be active against all bacteria tested. In general, extracts made from material grown in nature express better anti- bacterial activity comparing to those made from material grown in laboratory conditions. Some of the bacteria react the same to both extracts and some even better to the extracts made from axenically grown material.
Key words: Bryophytes, antibacterial activity, Atrichum undulatum, Marchantia polymorpha, Physcomitrella patens.