ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-006, March, 2019. © International Scholars Journals
Full Length Research Paper
Serum paraoxonase and arylesterase activities and oxidant status in patients with brucellosis
Hasan Karsen1*, Mustafa Kasım Karahocagil2, Hayrettin Akdeniz2, Mehmet Re at Ceylan3, Irfan Binici3, Sahbettin Selek4 and Hakim Celik4
1Department of Infectious Diseases and Clinical Microbiology, Harran Univesity School of Medicine, Sanliurfa, Turkey.
2Department of Infectious Diseases and Clinical Microbiology, Yuzuncu Yil Univesity School of Medicine, Sanliurfa, Turkey.
3Department of Infectious Diseases and Clinical Microbiology, Yuzuncu Yil Univesity School of Medicine, Van, Turkey.
4Department of Biochemistry, Harran Univesity School of Medicine, Sanliurfa, Turkey.
Accepted 23 January, 2019
Abstract
The aim of this study was to the investigate the effect of brucellosis infection on paraoxonase-1 (PON1) activity and oxidative status in patients with brucellosis. Sixty patients with brucellosis (47 acute, 13 subacute) and the 67 healthy controls were used in the study. Serum PON1 and arylesterase activities, total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), total free sulfhydryl (-SH), and lipid hydroperoxide (LOOH) groups were determined. In addition, HDL-cholesterol (HDL- C), LDL-cholesterol (LDL-C), total cholesterol (TC) and triglyceride (TG) were measured. Serum PON1 and arylesterase activities, total - SH group levels and TAC were significantly lower in brucellosis patients than controls (P<0.001). TOS, OSI and LOOH levels were significantly higher in brucellosis patients than controls (P<0.001). There were no significant differences between of serum LDL-C, TG and TC levels of patients subjects compared to controls (P > 0.05). Patients with brucellosis are exposed to potent oxidative stress and they have decreased PON1 activity. These predisposal factors might play a role in the pathogenesis for atherosclerosis in patients with brucellosis.
Key words: Brucellosis, PON1 activity, oxidative status, atherosclerosis.