ISSN 2756-3685
International Journal of Enology and Viticulture Vol. 7 (5), pp. 001-006, May, 2020. © International Scholars Journals
Full Length Research Paper
Cloning and functional characterization of a class III chitinase gene from grapevine: Inhibition of fungal growth by recombinant VvChiF III
S.A. Salami1*, A. Ebadi1, M.R. Naghavi2 and I.B. Dry3
1Department of Horticulture, Faculty of Horticultural Sciences and Plant Protection, University of Tehran, Karaj, Iran.
2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tehran, Karaj 31587, Iran.
3CSIRO Plant Industry, PO Box 350, Glen Osmond SA 5064, Australia.
Accepted 20 October, 2019
Abstract
To characterize the structure and function of chitinase genes, a class III chitinase gene (VvChiF III) was isolated from Vitis vinifera cv. Flame seedless. The VvChiF III open reading frame comprised 894 nucleotides with no introns and encoded a protein of 297 amino acids. The amino acid sequence encoded by VvChiF III showed a high identity to that of a class III chitinase isolated from V. vinifera cv. Koshu and to other acidic chitinase. Analysis of the VvChiF III amino acid sequence showed that this gene corresponds to the Glyco-hydro-18 super family that consisting of a signal peptide with the length of 25 amino acids. Purified VvChiF III showed chitinase activity toward the soluble substrate, glycolchitin and antifungal activity against Botrytis cinerea.
Key words: Vitis, chitinase genes, recombinant protein, antifungal activity, SNPs.