International Journal of Biochemistry and Biotechnology

ISSN 2169-3048

International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 14 (5), pp. 001-010, May, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Valorization of Pomegranate Peel for Invertase Production by a Marine Strain of Aspergillus terreus

Aliaa R. El-Shamy and Sherien M.M. Atalla

Department of Chemistry of Natural and Microbial Products, National Research Centre, Giza, Egypt

Accepted 20 March, 2025

Agricultural wastes were the low cost raw material for invertase production by fungi. The marine fungal strain Aspergillusterreus was characterized and phylogenetic analysis by 18S rRNA gene sequencing then evaluated for invertase production using 40 g/l of pomegranate peel (Pp) as substrate. Maximum invertase activity was 264.4 U/ml after 5 days incubation period. Two disks 6mm in diameter, pH 6.5 and 30 ◦C were the optimum conditions for invertase production. 3% of triton x100 as surfactant produce 281.2 U/ml .Increasing invertase production by addition of wheat bran ,EDTA and cysteine to pomegranate peel.

Keywords: marine fungi, invertase, pomegranate peel, optimization.