ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN: 2736-1624 Vol. 15 (1), pp. 001-011, January, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Activation of PPARγ by Cinnamon Extract Improves Glucose Regulation: Molecular and Physiological Insights
Rajesh Guptaa Sameer Walunja Shyam Awateb Roshan Kulkarnic Swati Joshic, Sushma Sabharwala , A. S. Padalkar b and Kalpana Joshib
aDepartment of Chemistry, Division of Biochemistry, University of Pune, Pune, India- 411007
bDepartment of Biotechnology, Sinhagad College of Engg, Pune, India-411041 c National Chemical Laboratory , Pune ,India- 411008
Accepted 18 December 2024
Cinnamon is one of the oldest spices in the world and widely used as an anti-diabetic medicine. We have investigated the effect of Cinnamon zeylanicum extracts on involvement of important coactivators like PGC1α and SRC1 in potentiation of PPARγ activity. Present study demonstrates that 50:50 ethanol aqueous extract of Cinnamomum zeylanicum bark contains specific PPARγ agonists that activate PPARγ in HEK293/T cell based reporter assay and also dose-dependently potentiate the extract mediated PPARγ activity in presence of co-activators like PGC1α and SRC1, overexpressed in the cells. Knockdown of PGC1α gene by specific shRNA against PGC1α, significantly reduced the transactivation potentiation effect of extract induced by coactivator. The ability to activate PPARγ receptor is also supported by its adipogenic potential observed in mouse 3T3-L1 adipogenesis assay. The CZE3 extract improved glucose tolerance in the 12 h fasted C57BL/6 male mice. In summary aqueous ethanolic extract of Cinnamomum zeylanicum exhibits part of its glucose lowering effect via activation of PPARγ and extract mediated PPARγ activity is increased upon overexpression of PGC1α and SRC1 in a cell based in vitro assay.
Key words: Cinnamon, Peroxisome proliferator activated receptors, Anti-hyperglycemic reporter assay, PGC1α, SRC1