ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (9), pp. 001-004, September, 2018. © International Scholars Journals
Full Length Research Paper
Utility of IS1245 -IS1311 based PCR typing system for Mycobacterium avium isolates obtained from clinical and environmental sources
G. P. S. Jadaun1, Prashant Upadhyay1, Zafar Ahmed1, Ram Das1, Deepti Parashar2, D. S. Chauhan1, V. D. Sharma1 and V. M. Katoch3*
1Department of Microbiology and Molecular Biology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases(Indian Council of Medical Research), P. O. Box 1101, Dr. M. Miyazaki Marg, Tajganj, Agra 282001, India.
2National Institute of Virology (ICMR) Pune, India.
3Department of Health Research, Ministry of Health and Family Welfare, Government of India and Director General, Indian Council of Medical Research Post Box No. 4911 Ansari Nagar, New Delhi-110029.
Accepted 14 May, 2018
Abstract
In the present study, twenty four Mycobacterium avium isolates obtained from clinical (sputum) and environmental (water and soil) samples were analyzed by a PCR typing method. Gel electrophoresis patterns showed that a single PCR reaction generated 21 profiles. Based on the relatedness of the PCR band patterns six isolates could be grouped in three clusters. Results have the implication that the PCR based system could be used as a simple and rapid method for molecular typing of M. avium isolates with high discriminatory index.
Key words: IS1245, IS1311, Mycobacterium avium, PCR typing.