ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 14 (1), pp. 001-005, January, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Comparative Analysis of Crude and Diluted Honey's Antibacterial Properties Against Wound Pathogens
W. Braide 1*, S.U.Oranusi1., C.K. Akaluka 1, R.N.Nwaoguikpe2., C.I. Akobundu1 and N.I. Peter-Ikechukwu3
1Department of Microbiology, Federal University of Technology, PMB 1526, Owerri, Imo State, Nigeria.
2Department of Biochemistry, Federal University of Technology, PMB 1526, Owerri, Imo State, Nigeria.
3Department of Food Science and Technology, Federal University of Technology, PMB 1526, Owerri, Imo State, Nigeria.
Accepted 05 October, 2024
Abstract
The antimicrobials activities of crude and diluted honey were determined against four clinical isolates from surface and deep wounds. Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli were isolated from wounds by routine microbiological methods. Kirby-Bauer’s disc diffusion method was employed in determining the susceptibility of bacterial isolates to different concentrations of honey. The results from the zone of inhibition obtained (in mm) showed that the growth of all isolates was completely inhibited by 20-100% honey concentrations. The minimum inhibitory concentrations (MIC) of honey for P. aeruginosa and S. aureus were at 10 % (v/v) while that of E.coli and K. pneumoniae were 20% (v/v). The degree of susceptibility of the wound isolates to honey was compared with that of ten commercially available antibiotic discs. The result obtained revealed that the susceptibility pattern of honey at 40% (v/v) compared favourably and better than amoxicillin, streptomycin, ceftriazone and erythromycin.
Keywords: Honey, antibacterial activities, wound bacteria.